Temporary Research Specialist

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Temporary Research Specialist

University Overview

The University of Pennsylvania, the largest private employer in Philadelphia, is a world-renowned leader in education, research, and innovation. This historic, Ivy League school consistently ranks among the top 10 universities in the annual U.S. News & World Report survey. Penn has 12 highly-regarded schools that provide opportunities for undergraduate, graduate and continuing education, all influenced by Penn's distinctive interdisciplinary approach to scholarship and learning. As an employer Penn has been ranked nationally on many occasions with the most recent award from Forbes who named Penn one of America's Best Employers By State in 2021.

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Posted Job Title
Temporary Research Specialist

Job Profile Title
Temporary Employee - Non-Exempt

Job Description Summary
Temporary Employee - Non-Exempt

The research specialist will assist in ophthalmic pathology and ocular surface related research.

Job Description

Temporary Employee - Non-Exempt

Execution of Visium Spatial Gene Expression (Visium), RNAscope, and related software analysis software - Visium enables RNA sequencing to be performed on tissue sections, thereby maintaining anatomical orientation. This technology combines throughput sequencing with high-resolution microscope to capture the expression pattern in tissue sections, down to a resolution of 1-10 cells. RNAscope is a novel in situ hybridization technique with a special probe designed to amplify target specific signals without background noise from non-specific signals. This technology is sensitive enough to detect a single transcript in a cell.

Image and statistical analysis, using a variety of programs and software that include Keyence microscope and imaging programs; Prism and/or Sigma Plot; R-Swirl; ImageJ; Photoshop adobe and illustrator; Excel and Powerpoint

Preparation and isolation of protein, RNA, and DNA from cell cultures, fresh tissue, frozen tissue, and formalin fixed paraffin embedded tissue - these specimens will undergo morphologic, proteomic, and genomic analysis.

Initiation and maintenance of human and mammalian corneal epithelial cells, which includes the construction of organotypic corneal epithelial models - these cells and models will be used to replicate epithelial wounds for subsequent protein and genomic analysis.

Execute western blots and subsequent analysis with either SDS-PAGE gels or Protein Simple Wes Western Blot technology.

Anesthetization, handling, wounding, imaging, and analysis of genetic mouse models - mice will be used for in vivo corneal wounding experiments.

Job Location - City, State
Philadelphia, Pennsylvania

Department / School
Perelman School of Medicine

Pay Range
$7.25 - $230.00

Affirmative Action Statement
Penn adheres to a policy that prohibits discrimination on the basis of race, color, sex, sexual orientation, gender identity, religion, creed, national or ethnic origin, citizenship status, age, disability, veteran status, or any other legally protected class.

Special Requirements
Background check may be required after a conditional job offer is made. Consideration of the background check will be tailored to the requirements of the job.

To apply, visit https://wd1.myworkdaysite.com/en-US/recruiting/upenn/careers-at-penn/job/Medical-School-John-Morgan-Bldg/Temporary-Research-Specialist_JR00042464

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